Researchers identify a genomic mechanism by which genes help distinguish a good number of scents

The human nose can distinguish one trillion different scents an extraordinary feat that requires 10 million specialized nerve cells, or neurons, in the nose, and a family of more than 400 dedicated genes. 


But precisely how these genes and neurons work in concert to pick out a particular scent has long puzzled scientists. 


This is in large part because the gene activity inside each neuron where each of these 10 million neurons only chooses to activate one of these hundreds of dedicated genes seemed far too simple to account for the sheer number of scents that the nose must parse.

But now, a Columbia study in mice has uncovered a striking resourcefulness: by rearranging itself in three-dimensional space, the genome coordinates the regulation of these genes in each neuron, thereby generating the biological diversity needed to detect the scents we experience.
The findings were published today in Nature.

“With today’s study, we’ve pinpointed a genomic mechanism by which a finite number of genes can ultimately help distinguish a seemingly near-infinite number of scents,” 

said Stavros Lomvardas, PhD, a principal investigator at Columbia’s Mortimer B. Zuckerman Mind Brain Behavior Institute and the paper’s senior author.

Smell, also known as olfaction, is mind-bogglingly complex. The receptors in our noses must not only identify a scent, but also gauge how strong it is, scan our memories to determine whether it has been encountered before, and determine if it is pleasing or toxic.

Olfactory receptor neurons, specialized nerve cells that snake from the nose to the brain, make all this possible.

And though each neuron contains the full suite of the 400 dedicated olfactory receptor genes, only one of these genes is active in each neuron.

 Adding to the confusion: the gene that is active appears randomly chosen, and differs from neuron to neuron.

This unusual pattern of gene activity is known as the “one gene per neuron” rule, and has long been a focus of study by scientists such as Dr. Lomvardas. Indeed, deciphering how each olfactory receptor neuron manages to activate only one of these genes and how this process results in such a finely tuned sense of smell remained mysterious for decades.

“In mice, olfactory receptor genes are scattered across the genome at about 60 different locations on different chromosomes that are quite far apart from each other,” 

said Kevin Monahan, PhD, a postdoctoral research scientist in the Lomvardas lab and the paper’s co-first author.

Mice have about 1,000 olfactory receptor genes, more than twice that of humans, potentially indicative of a superior sense of smell.

Traditionally, it has been thought that genes located on different chromosomes rarely, if ever, interacted with each other.

By employing a new genomic sequencing technique called in situ Hi-C, Dr. Lomvardas and his team recently revealed that the chromosomes interacted much more frequently than expected.

“In situ Hi-C is revolutionary in large part because it allows us to map, in 3D, the entire genome inside a living cell,” 

said Adan Horta, PhD, a recently graduated doctoral candidate in the Lomvardas lab and the paper’s co-first author.

 “This gives us a snapshot of the genome at a particular point in time.”

Snapshots taken by the researchers showed clusters of olfactory receptor genes, located on different chromosomes, physically moving toward each before choosing an olfactory receptor gene.

Soon after these genes huddled together, another type of genetic element known as enhancers clustered in a separate 3D compartment.

Enhancers are not themselves genes but regulate the activity of genes.

“We previously discovered a group of enhancers, we named the Greek Islands, located near the various olfactory receptor genes,”

said Dr. Horta.

 “This work showed that these enhancers create hotspots of activity to regulate the “chosen” olfactory receptor gene.

The team also found that the protein Ldb1 plays a key role in this process.

It holds the Greek Islands together, allowing them to switch on a specific olfactory receptor gene that then as a team interpret the particular scent at hand.

“These teams of genes endow the olfactory system with the ability to respond in diverse ways,” 

said Dr. Monahan.

“The flexibility of this process could help to explain how we easily learn and remember new smells.”

Though specific to olfaction, the researchers’ findings could have implications for other areas of biology in which inter-chromosome interactions play a role.

The Zuckerman Institute at Columbia University